Composite

Part:BBa_K5366021:Design

Designed by: Lin Li   Group: iGEM24_NJTECH-CHINA-A   (2024-09-27)


T7 promoter-RBS-UxaE-6xHis -T7 termonator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1516
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 540
    Illegal BglII site found at 1302
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 294
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We ensured that the forward and reverse primer had 20 bp of homology to the plasmid.We needed to purify the UxaE protein, so we attached the 6×His tag after UxaE.

Source

Thermotoga petrophila

References